检查 有助于确认免疫荧光抗体测定 (IFA) 或在两种细菌都流行的地区（例如大西洋中部或东北部各州）鉴别查菲埃立克体和嗜吞噬细胞无形体感染。[54]Horowitz HW, Aguero-Rosenfeld ME, McKenna DF, et al. Clinical and laboratory spectrum of culture-proven human granulocytic ehrlichiosis: comparison with culture-negative cases. Clin Infect Dis. 1998;27:1314-1317.http://www.ncbi.nlm.nih.gov/pubmed/9827289?tool=bestpractice.com[92]Brouqui P, Dumler JS, Raoult D, et al. Antigenic characterization of ehrlichiae: protein immunoblotting of Ehrlichia canis, Ehrlichia sennetsu, and Ehrlichia risticii. J Clin Microbiol. 1992 May;30(5):1062-6.http://jcm.asm.org/cgi/reprint/30/5/1062http://www.ncbi.nlm.nih.gov/pubmed/1583101?tool=bestpractice.com[93]Chen SM, Dumler JS, Feng HM, et al. Identification of the antigenic constituents of Ehrlichia chaffeensis. Am J Trop Med Hyg. 1994 Jan;50(1):52-8.http://www.ncbi.nlm.nih.gov/pubmed/8304572?tool=bestpractice.com[94]Chen SM, Cullman LC, Walker DH. Western immunoblotting analysis of the antibody responses of patients with human monocytotropic ehrlichiosis to different strains of Ehrlichia chaffeensis and Ehrlichia canis. Clin Diagn Lab Immunol. 997 Nov;4(6):731-5.http://cvi.asm.org/cgi/reprint/4/6/731http://www.ncbi.nlm.nih.gov/pubmed/9384299?tool=bestpractice.com[95]Wong SJ, Brady GS, Dumler JS. Serological responses to Ehrlichia equi, Ehrlichia chaffeensis, and Borrelia burgdorferi in patients from New York State. J Clin Microbiol. 1997 Sep;35(9):2198-205.http://jcm.asm.org/cgi/reprint/35/9/2198http://www.ncbi.nlm.nih.gov/pubmed/9276387?tool=bestpractice.com[96]Yu XJ, Crocquet-Valdes P, Cullman LC, et al. The recombinant 120-kilodalton protein of Ehrlichia chaffeensis, a potential diagnostic tool. J Clin Microbiol. 1996 Nov;34(11):2853-5.http://jcm.asm.org/cgi/reprint/34/11/2853http://www.ncbi.nlm.nih.gov/pubmed/8897200?tool=bestpractice.com[97]Unver A, Felek S, Paddock CD, et al. Western blot analysis of sera reactive to human monocytic ehrlichiosis and human granulocytic ehrlichiosis agents. J Clin Microbiol. 2001 Nov;39(11):3982-6.http://jcm.asm.org/cgi/content/full/39/11/3982http://www.ncbi.nlm.nih.gov/pubmed/11682518?tool=bestpractice.com[98]Tajima T, Zhi N, Lin Q, et al. Comparison of two recombinant major outer membrane proteins of the human granulocytic ehrlichiosis agent for use in an enzyme-linked immunosorbent assay. Clin Diagn Lab Immunol. 2000 Jul;7(4):652-7.http://cvi.asm.org/cgi/content/full/7/4/652http://www.ncbi.nlm.nih.gov/pubmed/10882667?tool=bestpractice.com[99]Luo T, Zhang X, Nicholson WL, et al. Molecular characterization of antibody epitopes of Ehrlichia chaffeensis ankyrin protein 200 and tandem repeat protein 47 and evaluation of synthetic immunodeterminants for serodiagnosis of human monocytotropic ehrlichiosis. Clin Vaccine Immunol. 2010 Jan;17(1):87-97.http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2812101/?tool=pubmedhttp://www.ncbi.nlm.nih.gov/pubmed/19955322?tool=bestpractice.com
分析灵敏度在理论上高于 IFA，但尚未对其系统评估。
仅在高度专业化的研究性实验室可用。
[Figure caption and citation for the preceding image starts]: 外周血涂片显示，在一个多形核中性粒细胞中，存在细胞质内桑葚状（箭头所示）嗜吞噬细胞无形体（吉姆萨染色，1000x）承蒙 J. Stephen Dumler 医学博士提供 [Citation ends].